Extended Storage of Microbial Cultures and Culturing Anaerobic Bacteria with Plastic Pouches

Simple and convenient method for culturing anaerobic bacteria.

A simple and convenient method for culturing anaerobic bacteria is described. Cultures can be grown in commercially available flasks normally used for preparation of sterile external solutions. A special disposable rubber flask closure maintains anaerobic conditions in the flask after autoclaving. Growth of a variety of anaerobic oral bacteria was comparable to that obtained after anaerobic inc...

Culturing Aerobic and Anaerobic Bacteria and Mammalian Cells with a Microfluidic Differential Oxygenator

In this manuscript, we report on the culture of anaerobic and aerobic species within a disposable multilayer polydimethylsiloxane (PDMS) microfluidic device with an integrated differential oxygenator. A gas-filled microchannel network functioning as an oxygen-nitrogen mixer generates differential oxygen concentration. By controlling the relative flow rate of the oxygen and nitrogen input gases,...

14-CO-2 trapping assembly for monitoring anaerobic microbial cultures.

This note describes a "4CO2 trapping assembly for anaerobic microbial cultures which is easily operated, cleaned, and assembled, and requires no specialized glassware. The assembly in operation has the advantages of providing a means of: purging an anaerobic microbial culture with a gas containing C02; monitoring of '4CO2 produced while the assembly is operating; trapping 14Cvolatilized non-1"C...

Routine Testing for Anaerobic Bacteria in Cerebrospinal Fluid Cultures Improves

Mastoiditis and Gradenigo’s Syndrome with anaerobic bacteria

UNLABELLED BACKGROUND Gradenigo's syndrome is a rare disease, which is characterized by the triad of the following conditions: suppurative otitis media, pain in the distribution of the first and the second division of trigeminal nerve, and abducens nerve palsy. The full triad may often not be present, but can develop if the condition is not treated correctly. CASE PRESENTATION We report a ...